"On the Nature of the Process of Fertilization and the Artificial Production of Normal Larvae (Plutei) From the Unfertilized Eggs of the Sea Urchin" (1899), by Jacques Loeb

Jacques Loeb [4] developed procedures to make embryos from unfertilized sea urchin [5] eggs in 1899. Loeb called the procedures ?artificial parthenogenesis," and he introduced them and his results in ?On the Nature of the Process of Fertilization and the Artificial Production of Norma Larvae (Plutei) from the Unfertilized Eggs of the Sea Urchin? in an 1899 issue of The American Journal of Physiology. In 1900 Loeb elaborated on his experiments. Following those publications, however, he discovered he had used inaccurately labeled salts and redid his experiments to determine the correct amount of salts needed for artificial parthenogenesis [6].